{"id":256,"date":"2025-11-05T19:42:45","date_gmt":"2025-11-05T19:42:45","guid":{"rendered":"https:\/\/pressbooks.hcfl.edu\/bio1ss\/?post_type=chapter&p=256"},"modified":"2026-09-10T17:06:19","modified_gmt":"2026-09-10T17:06:19","slug":"lab-8-cellular-respiration","status":"publish","type":"chapter","link":"https:\/\/pressbooks.hcfl.edu\/bio1ss\/chapter\/lab-8-cellular-respiration\/","title":{"raw":"Lab 8: Cellular Respiration","rendered":"Lab 8: Cellular Respiration"},"content":{"raw":"

Laboratory 8: Cellular Respiration<\/span><\/h1>\r\n
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Learning Objectives<\/h2>\r\nAfter completing this laboratory exercise, you should be able to:\r\n
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  1. Explain the role of cellular respiration in ATP production.<\/li>\r\n \t
  2. Measure oxygen consumption as evidence of cellular respiration.<\/li>\r\n \t
  3. Compare respiration rates in germinating and non-germinating beans.<\/li>\r\n \t
  4. Investigate how different sugars affect yeast respiration.<\/li>\r\n \t
  5. Explain how enzyme specificity influences substrate utilization.<\/li>\r\n \t
  6. Analyze and interpret respiration data using graphs and trendlines.<\/li>\r\n \t
  7. Calculate respiration rates using the slope of an oxygen-consumption graph.<\/li>\r\n<\/ol>\r\n\r\n
    \r\n\r\n

    Key Terms<\/h1>\r\n
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    \r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
    Term<\/th>\r\nDefinition<\/th>\r\n<\/tr>\r\n
    Cellular Respiration<\/td>\r\nProcess by which cells convert energy stored in food molecules into ATP.<\/td>\r\n<\/tr>\r\n
    Aerobic Respiration<\/td>\r\nCellular respiration that requires oxygen.<\/td>\r\n<\/tr>\r\n
    Fermentation<\/td>\r\nATP production without oxygen.<\/td>\r\n<\/tr>\r\n
    Metabolism<\/td>\r\nThe sum of all chemical reactions occurring in a cell or organism.<\/td>\r\n<\/tr>\r\n
    Respiration Rate<\/td>\r\nRate at which oxygen is consumed or carbon dioxide is produced.<\/td>\r\n<\/tr>\r\n
    Substrate<\/td>\r\nMolecule used in a metabolic reaction.<\/td>\r\n<\/tr>\r\n
    Enzyme Specificity<\/td>\r\nAbility of enzymes to interact with certain substrates.<\/td>\r\n<\/tr>\r\n
    Oxidation-Reduction (Redox) Reaction<\/td>\r\nChemical reaction involving electron transfer.<\/td>\r\n<\/tr>\r\n
    Methylene Blue<\/td>\r\nRedox indicator that is blue when oxidized and colorless when reduced.<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n\r\n
    \r\n\r\n

    Introduction<\/h1>\r\nCellular respiration is the process by which cells convert the chemical energy stored in food molecules into ATP, the usable energy currency of the cell.\r\n\r\nThe overall equation for aerobic cellular respiration is:\r\n\r\nC<\/mi>6<\/mn><\/msub>H<\/mi>12<\/mn><\/msub>O<\/mi>6<\/mn><\/msub>+<\/mo>6<\/mn>O<\/mi>2<\/mn><\/msub>\u2192<\/mo>6<\/mn>C<\/mi>O<\/mi>2<\/mn><\/msub>+<\/mo>6<\/mn>H<\/mi>2<\/mn><\/msub>O<\/mi>+<\/mo>A<\/mi>T<\/mi>P<\/mi><\/mrow>C_6H_{12}O_6 + 6O_2 \\rightarrow 6CO_2 + 6H_2O + ATP<\/annotation><\/semantics><\/math><\/span>\r\n<\/span><\/span>During cellular respiration:\r\n